Grade 10 general science – Microorganisms Quiz

1. Which of the following is NOT a type of microorganism?

Bacteria
Insects such as mosquitoes
Viruses
Fungi
Explanation:

Insects are multicellular animals visible to the naked eye and are not classified as microorganisms. Bacteria, viruses and fungi can be microscopic.

2. What is the main way most bacteria reproduce?

Binary fission (splitting into two)
Sexual reproduction with gametes
Spore formation
Budding like yeast
Explanation:

Most bacteria reproduce asexually by binary fission, where one cell divides into two genetically similar cells. Some microbes like fungi use spores and some yeasts bud, but binary fission is typical for bacteria.

3. Which statement correctly distinguishes viruses from bacteria?

Bacteria are much larger than viruses and can live independently, viruses need host cells to replicate
Viruses can reproduce on their own outside a host cell, bacteria cannot
Bacteria always cause disease while viruses never do
Viruses are visible with a light microscope but bacteria are not
Explanation:

Bacteria are generally larger and can grow and reproduce independently, whereas viruses are much smaller and require a host cell's machinery to replicate.

4. Which microorganism is the cause of malaria?

A virus (Influenza)
A protozoan (Plasmodium)
A bacterium (Vibrio)
A fungus (Aspergillus)
Explanation:

Malaria is caused by protozoan parasites of the genus Plasmodium, which are transmitted to humans by Anopheles mosquitoes.

5. Which of the following diseases is caused by a bacterium?

Common cold
HIV/AIDS
Cholera
Measles
Explanation:

Cholera is caused by the bacterium Vibrio cholerae. Measles and the common cold are viral, and HIV/AIDS is caused by the human immunodeficiency virus.

6. Which practice helps make drinking milk safer by killing many harmful microorganisms?

Adding sugar to milk
Pasteurization (heating milk briefly)
Letting milk sit at room temperature
Filtering milk through cloth only
Explanation:

Pasteurization involves heating milk to a specific temperature briefly to kill many harmful microbes while preserving nutritional quality; simply letting milk sit or filtering through cloth does not reliably eliminate pathogens.

7. Which microorganisms fix atmospheric nitrogen in root nodules of beans and other legumes?

Green algae
Fungi like Penicillium
Rhizobium bacteria
Influenza viruses
Explanation:

Rhizobium bacteria form symbiotic relationships with legume roots and convert atmospheric nitrogen into forms the plant can use, improving soil fertility.

8. Why are antibiotics not effective against viral infections?

Antibiotics always cause viruses to mutate faster
Viruses are too large for antibiotics to enter
Antibiotics target bacterial structures/processes not found in viruses
Viruses live only in water and antibiotics dissolve there
Explanation:

Antibiotics interfere with bacterial cell walls, protein synthesis or other bacterial-specific processes; viruses lack these structures and use host cells to replicate, so antibiotics do not work on them.

9. Which Kenyan food or product commonly involves beneficial microorganisms during preparation?

Fresh roasted maize that is not fermented
Uji (fermented porridge)
Raw, unprocessed meat left at room temperature
Boiled tea without fermentation
Explanation:

Uji is often prepared by fermentation using naturally occurring bacteria or yeasts, which improve flavor and sometimes digestibility. Fresh roasted maize or boiled tea don’t rely on microbial fermentation.

10. Which microorganism group includes yeasts used in bread making?

Bacteria
Fungi
Viruses
Protozoa
Explanation:

Yeasts are single-celled fungi (e.g., Saccharomyces cerevisiae) that ferment sugars to produce carbon dioxide and alcohol, causing bread to rise.

11. What is a safe household method to reduce microbes in drinking water?

Storing water in any open container
Adding sugar to the water
Boiling the water for at least one minute
Leaving water in sunlight for several hours without a cover
Explanation:

Boiling water kills most disease-causing microorganisms. Leaving water uncovered or adding sugar does not make it safe.

12. Which microorganism causes the disease tuberculosis (TB)?

A protozoan (Giardia)
A fungus (Candida)
A virus (Rhinovirus)
A bacterium (Mycobacterium tuberculosis)
Explanation:

Tuberculosis is caused by the bacterium Mycobacterium tuberculosis and is transmitted mainly via droplets when an infected person coughs or sneezes.

13. In a classroom microscope practical, which stain is commonly used to see bacteria more clearly by increasing contrast?

Crystal violet or simple staining
Carmine for animal tissues only
No stain, because bacteria are always colored
Iodine for plant cell walls only
Explanation:

Simple stains like crystal violet color bacterial cells so they are easier to see under a light microscope. Without staining bacteria are often hard to see because they are nearly transparent.

14. Which role do decomposer microorganisms play in the environment?

Make soil sterile and remove all nutrients
Break down dead organic matter and return nutrients to the soil
Prevent plants from taking up water
Cause immediate extinction of other species
Explanation:

Decomposer bacteria and fungi break down dead plants and animals, releasing nutrients that plants can reuse, maintaining ecosystem nutrient cycles.

15. What is the main purpose of the Gram stain in microbiology?

Detect viruses in a sample
Sterilize the sample before viewing
Differentiate bacteria by cell wall structure into Gram-positive and Gram-negative
Measure bacterial motility
Explanation:

The Gram stain differentiates bacteria based on cell wall properties: Gram-positive retain crystal violet and appear purple, while Gram-negative do not and appear pink after counterstaining.

16. Which microorganism is responsible for athlete's foot and ringworm?

Fungi (dermatophytes)
Protozoa
Bacteria
Viruses
Explanation:

Athlete's foot and ringworm are caused by fungi called dermatophytes that infect skin, hair and nails.

17. Algae in freshwater lakes are important because they:

Produce oxygen through photosynthesis and form the base of the food web
Cause soil erosion on lake shores
Consume large fish as their main food
Stop sunlight from reaching beach sand
Explanation:

Photosynthetic algae produce oxygen and serve as primary producers that support aquatic food chains.

18. Why is handwashing with soap effective at reducing disease spread?

Soap removes and helps destroy microbes on the skin by breaking down oils and disrupting membranes
Soap makes the skin waterproof against bacteria
Soap causes immediate immunity to diseases
Soap feeds microbes so they stay on the hands
Explanation:

Soap emulsifies oils that trap microbes and can disrupt membranes of some microbes, allowing them to be rinsed away, reducing disease transmission.

19. Which of the following is a viral disease that has vaccines available in Kenya?

Measles
Ringworm
Cholera
Giardiasis
Explanation:

Measles is caused by a virus and effective vaccines are part of routine immunisation programs. Cholera is bacterial, ringworm is fungal, and giardiasis is protozoan.

20. What is the role of fermented foods in relation to microorganisms?

Fermentation removes all vitamins from food
Microbes always spoil fermented foods making them unsafe
Fermentation makes food heavier and toxic
Beneficial microbes transform food (flavour, preservation, nutrients)
Explanation:

During fermentation, yeasts and bacteria produce acids, alcohols and enzymes that preserve food, change flavour and sometimes increase nutritional value.

21. Which method is used in laboratories to grow and study bacteria safely?

Leaving samples open on a bench to collect dust
Mixing bacteria with household cleaning agents for fun
Growing bacteria only in drinking water at home
Culture on sterile nutrient agar plates in controlled conditions
Explanation:

Lab cultures on sterile agar allow scientists to grow, isolate and study bacteria safely under controlled conditions with proper aseptic technique.

22. A student observes small, moving single-celled organisms in pond water under a microscope. These are most likely:

Large insects
Protozoa
Fungi
Viruses
Explanation:

Protozoa are single-celled, often motile organisms commonly seen moving in pond water under a light microscope; viruses are too small to see with a basic light microscope.

23. Which practice contributes to antibiotic resistance in communities?

Boiling water before drinking
Washing hands regularly with soap
Vaccinating children according to schedule
Using antibiotics unnecessarily or not finishing prescribed courses
Explanation:

Misuse of antibiotics (overuse, underuse or incomplete courses) allows bacteria to survive and develop resistance; good hygiene and vaccination help prevent infections and reduce antibiotic use.

24. During a practical, how should a student dispose of used microbial culture plates to be safe?

Throw them into a classroom dustbin with food waste
Place them in a labelled biohazard container for autoclaving or appropriate disposal
Wash them in the sink with cold water only
Take them home to show friends
Explanation:

Used culture plates may contain live microbes and must be decontaminated (e.g., autoclaved) and discarded as biohazardous waste following safety protocols to prevent contamination and spread.

25. Which statement best describes the size ranking from smallest to largest among these microorganisms?

Fungi, protozoa, bacteria, viruses
Protozoa, viruses, bacteria, fungi
Bacteria, viruses, fungi, protozoa
Viruses, bacteria, protozoa, fungi
Explanation:

Viruses are the smallest, followed by bacteria, then protozoa (single-celled eukaryotes), and fungi which are often larger (including multicellular forms).

26. Which statement best describes microorganisms?

They can easily be seen without a microscope like insects
They only live in hospitals and labs
They are too small to be seen with the naked eye
They are always multicellular like plants
Explanation:

Microorganisms are generally microscopic (bacteria, viruses, protozoa, many fungi) and require a microscope to be seen; some fungi produce larger structures but most microbes are too small for the naked eye.

27. Which of the following is a virus?

Yeast
Influenza
Escherichia coli
Amoeba
Explanation:

Influenza is caused by an influenza virus; E. coli is a bacterium, yeast is a fungus, and amoeba are protozoa.

28. Which microorganism causes malaria?

Trypanosoma brucei
Plasmodium falciparum (a protozoan)
Anopheles mosquito (a vector)
Vibrio cholerae
Explanation:

Malaria is caused by Plasmodium species (a protozoan); the Anopheles mosquito transmits the parasite but is not the microorganism that causes the disease.

29. Which microorganism commonly fixes nitrogen in the soil for legume crops in Kenya?

Influenza virus
Paramecium protozoan
Rhizobium bacteria
Aspergillus fungus
Explanation:

Rhizobium bacteria form nodules on legume roots and convert atmospheric nitrogen into forms plants can use, improving soil fertility.

30. What process is used to make milk safe by killing many harmful microorganisms while keeping milk nutritious?

Fermentation
Freezing
Simple filtration through cloth
Pasteurization
Explanation:

Pasteurization uses controlled heating to kill many pathogens in milk without severely affecting its nutritional value; filtration or freezing do not reliably kill pathogens.

31. Antibiotics are NOT effective in treating which of the following?

Influenza (a viral infection)
Streptococcal throat infection
Urinary tract infection caused by bacteria
Bacterial pneumonia
Explanation:

Antibiotics target bacteria and have no effect on viruses such as influenza; viral infections require antiviral treatment or supportive care.

32. Which structure is present in bacteria but absent in viruses?

Protein coat
Genetic material
Need for a host cell to reproduce
Cell membrane
Explanation:

Bacteria are cellular and have a cell membrane (and often a cell wall); viruses are not cells and lack a cell membrane, having only a protein coat and genetic material.

33. Which microorganism is used in baking bread to make dough rise?

Escherichia coli
Paramecium
Rhizobium
Yeast (Saccharomyces)
Explanation:

Baker's yeast ferments sugars producing carbon dioxide that makes dough rise; E. coli and Rhizobium are bacteria, Paramecium is a protozoan.

34. What is binary fission?

Sexual reproduction between two cells
A mutation process that kills cells
The production of spores by fungi
A form of asexual reproduction where one cell divides into two
Explanation:

Binary fission is how many bacteria reproduce asexually: the cell copies its DNA and splits into two genetically similar daughter cells.

35. Which method is commonly used in laboratories to sterilize equipment using steam under pressure?

Sun drying
Autoclaving
Freezing
Simple cloth filtration
Explanation:

Autoclaving uses high-pressure steam to reliably sterilize instruments by killing bacteria, spores and many viruses; sun drying, freezing or cloth filtration do not sterilize.

36. What is the main role of decomposer microorganisms in the environment?

Produce oxygen by photosynthesis
Carry oxygen in animal blood
Protect plants from disease by living inside leaves
Break down dead matter and recycle nutrients
Explanation:

Decomposers (bacteria and fungi) break down dead plants and animals, returning nutrients to the soil for use by living organisms.

37. Which of the following is a beneficial bacterium commonly found in the human gut and in fermented foods?

Plasmodium
Influenza virus
Salmonella
Lactobacillus
Explanation:

Lactobacillus species are probiotic bacteria that help digestion and are used in fermented foods like yoghurt; Salmonella is a pathogen, influenza a virus, Plasmodium a protozoan.

38. Which disease is caused by the bacterium Vibrio cholerae?

Malaria
Cholera
Ringworm
Measles
Explanation:

Vibrio cholerae is the bacterium that causes cholera, a severe diarrhoeal disease often spread via contaminated water.

39. At the household level in Kenya, what is a reliable way to make water safe to drink from an uncertain source?

Adding sugar to the water
Boiling the water for at least one minute
Filtering once through a tea cloth only
Storing the water in a warm place
Explanation:

Boiling water kills most disease-causing microorganisms; adding sugar or simple cloth filtration do not reliably make water safe.

40. Which type of microorganism causes ringworm (a skin infection), common in schools?

Bacterium
Virus
Fungus
Protozoan
Explanation:

Ringworm is caused by dermatophyte fungi; it is not caused by bacteria, viruses or protozoa.

41. Why should students wear goggles and gloves when working with microbes in a school lab?

To protect themselves from accidental contact and contamination
To keep the microbes warm
Because microbes are visible and might stain clothes
To look professional even if not needed
Explanation:

Goggles and gloves reduce the risk of infection or contamination from accidental spills, splashes or touching contaminated surfaces during experiments.

42. Which statement about viruses is correct?

Viruses require a host cell to reproduce
Viruses always contain both DNA and RNA
Viruses are larger than most bacteria
Viruses can grow independently like bacteria
Explanation:

Viruses are obligate parasites that must enter host cells to replicate; they cannot reproduce independently. They are generally smaller than bacteria and usually carry either DNA or RNA, not both.

43. Which practice reduces bacterial growth in perishable food stored at home?

Storing food in moist, warm containers
Refrigeration at low temperature
Leaving food at room temperature for long periods
Keeping food in direct sunlight
Explanation:

Lower temperatures slow the metabolism and reproduction of many bacteria, helping to keep food safe longer; warmth and moisture promote bacterial growth.

44. Arrange these microorganisms from smallest to largest: virus, bacteria, protozoan, fungus.

Fungus, protozoan, bacteria, virus
Bacteria, virus, fungus, protozoan
Virus, bacteria, protozoan, fungus
Protozoan, virus, bacteria, fungus
Explanation:

Generally viruses are smallest, bacteria are larger, protozoa (single-celled eukaryotes) are larger still, and many fungi have the largest cells or multicellular structures.

45. Which staining method is commonly used to classify bacteria as Gram-positive or Gram-negative?

Gram staining
Negative staining with oil
Simple staining with iodine only
Endospore staining
Explanation:

Gram staining differentiates bacteria by cell wall structure into Gram-positive and Gram-negative, which helps guide treatment and identification.

46. Which microorganism causes tuberculosis (TB)?

Streptococcus pneumoniae
Plasmodium falciparum
Influenza virus
Mycobacterium tuberculosis (a bacterium)
Explanation:

Tuberculosis is caused by the bacterium Mycobacterium tuberculosis; other options cause different diseases (pneumonia, flu, malaria).

47. What is the main purpose of vaccination?

To cure infections after they occur
To sterilize all microbes in the environment
To stimulate the immune system to develop protection against a specific disease
To give antibiotics to the body
Explanation:

Vaccines expose the immune system to safe parts of a pathogen or weakened forms so the body builds memory cells and can fight the disease if exposed later.

48. Which technique is used to grow bacteria for observation in a school laboratory?

Culturing on nutrient agar plates
Storing the sample in a sealed plastic bag at room temperature
Boiling the sample before observing
Looking directly at food without any preparation
Explanation:

Culturing on nutrient agar provides bacteria with nutrients and a surface to grow, allowing observation and study under controlled conditions; the other methods are not appropriate for growing and observing bacteria.

49. Which microorganism is most commonly associated with dental tooth decay?

Plasmodium falciparum
Rhinovirus
Lactobacillus plantarum (beneficial only)
Streptococcus mutans
Explanation:

Streptococcus mutans is a bacterium that metabolizes sugars to produce acids that demineralize tooth enamel, leading to cavities.

50. Which characteristic is NOT true of fungi?

They can reproduce by spores
They photosynthesize like green plants
They often have cell walls made of chitin
Many act as decomposers of organic matter
Explanation:

Fungi do not photosynthesize; they obtain nutrients by absorbing organic matter. They do have chitin cell walls, reproduce by spores, and many are decomposers.

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